Multiphoton Microscopy
نویسندگان
چکیده
منابع مشابه
Multiphoton Microscopy
Multi-Photon Fluorescence Microscopy is a relatively novel imaging technique in cell biology. It relies on the quasi-simultaneous absorption of two or more photons (of either the same or different energy) by a molecule. With normal fluorescence microscopy, one cannot resolve deep structures within a specimen because of large depth of focus. An enhanced method, the confocal microscopy [1], allow...
متن کاملMultiphoton fluorescence microscopy.
Multiphoton fluorescence microscopy has now become a relatively common tool among biophysicists and biologists. The intrinsic sectioning achievable by multiphoton excitation provides a simple means to excite a small volume inside cells and tissues. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal c...
متن کاملMultifocal multiphoton microscopy.
We present a real-time, direct-view multiphoton excitation fluorescence microscope that provides three-dimensional imaging at high resolution. Using a rotating microlens disk, we split the near-infrared light of a mode-locked titanium-sapphire laser into an array of beams that are transformed into an array of high-aperture foci at the object. We typically scan at 225 frames per second and image...
متن کاملMultiphoton plasmon-resonance microscopy.
A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal. This enhanced signal is measured to obtain a depth-resolved image in a laser scanning microscop...
متن کاملMultiphoton Fluorescence Microscopy
The human body is a constant flux of thousands of chemical/biological interactions and processes connecting molecules, cells, organs, and fluids, throughout the brain, body, and nervous system. Up until recently it was thought that all these interactions operated in a linear sequence, passing on information much like a runner passing the baton to the next runner. However, the latest findings in...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2003
ISSN: 1083-3668
DOI: 10.1117/1.1594726